Buy 7-ADD (7-Aminoactinomycin D) For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

What is 7-ADD (7-Aminoactinomycin D)

7-Aminoactinomycin D (7-AAD) is a fluorescent chemical compound widely used in molecular biology and flow cytometry for DNA staining and cell viability analysis. It intercalates with double-stranded DNA, preferentially binding at GC-rich regions, resulting in strong red fluorescence emission. 7-AAD is membrane impermeant, allowing it to selectively stain dead or membrane-compromised cells in mixed populations. It is a valuable tool for assessing cell cycle, apoptosis, and viability in research contexts. This compound is intended strictly for laboratory use and not for therapeutic or diagnostic human consumption.

Technical Information of 7-ADD (7-Aminoactinomycin D)

Typical Research Applications of 7-ADD

7-AAD is extensively used in cell biology applications to discriminate live and dead cells by selectively staining cells with compromised membranes. It is widely utilized in flow cytometry for viability assays, apoptosis detection, and cell cycle analysis. Additionally, 7-AAD is applied in fluorescence microscopy and chromosome banding studies due to its preferential DNA binding and fluorescence properties.

Safety and Handling Information for 7-ADD

Handle with gloves, lab coat, and eye protection in a well-ventilated area or fume hood. Avoid inhalation, ingestion, and skin or eye contact. In case of exposure, flush with water and seek medical attention. Store in airtight, light-protected containers at -20°C.

Ordering & Shipping Summary for 7-ADD

Available as lyophilized powder or solution, shipped with temperature control and appropriate documentation. Certificates of analysis confirm compound purity and quality. Shipping complies with regulations for laboratory chemicals.

Storage Conditions for 7-ADD

Store lyophilized powder at -20°C, protected from light and moisture to maintain stability. Prepare fresh working solutions as recommended and store temporarily on ice or refrigerated conditions.

Alternatives to 7-ADD

Alternatives include other DNA-binding fluorescent stains like Propidium Iodide, SYTOX dyes, or DAPI depending on specific fluorescence requirements and experimental conditions.

FAQ About 7-ADD

• Is 7-ADD suitable for human diagnostic use?
  No, 7-AAD is solely for laboratory research use.

• What purity is guaranteed?
  Purity is ≥97%, confirmed by HPLC.

• How should the compound be stored?
  Store at -20°C, protected from light in airtight containers.

• Can 7-ADD be used in live cell staining?
  It only stains dead or membrane-compromised cells; not suitable for staining live cells.

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